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Examples of the the word, plasmid , in a Sentence Context

The word ( plasmid ), is the 19489 most frequently used in English word vocabulary

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  1. Speeds (slowest to fastest: nicked or open circular, linear,or super coiled, plasmid ,). Visualization: ethidium bromide (EBR) and dyes The most common dye used
  2. Cells. This enables the transfer of plasmid s between the bacteria. An exchanged, plasmid ,can code for new functions, e. g., antibiotic resistance. The plus is made up
  3. Decrease the resolution (above about 5 to 8 V/cm). Conformations of a DNA, plasmid ,that has not been cut with a restriction enzyme will move with different speeds
  4. Of TEM or SHV lineage have recently been described. The SBS are frequently, plasmid ,encoded. Plasmids responsible for ESL production frequently carry genes
  5. Attaches to recipient cell and brings the two cells together. 3- The mobile, plasmid ,is nicked and a single strand of DNA is then transferred to the recipient cell.
  6. The presence of a plasmid in a strain of B. thuringiensis and suggested the, plasmid ,'s involvement in endospore and crystal formation. B. thuringiensis is closely
  7. Bacteria. In molecular biology application, this allows for its isolation from, plasmid ,DNA by centrifugation of lased bacteria and pelleting of the membranes (and
  8. Recombinant DNA technologies involving the insertion of a DNA sequence into a, plasmid ,or the genetic material of another organism. Bacterial colonies (E. coli) can
  9. Available for such uses. The gene to be replicated is inserted into copies of a, plasmid ,containing genes that make cells resistant to particular antibiotics and a
  10. Only bacteria that take up copies of the plasmid survive, since the, plasmid ,makes them resistant. In particular, the protecting genes are expressed (used
  11. Have promoter regions upstream the replication/transcription start site. This, plasmid ,can be inserted into either bacterial or animal cells. Introducing DNA into
  12. Associated with conjugation, a mechanism of horizontal gene transfer. The term, plasmid ,was first introduced by the American molecular biologist Joshua Lederberg in
  13. In most strains of B. thuringiensis, the cry genes are located on the, plasmid , Cry toxins have specific activities against insect species of the orders
  14. Recombination or ballistics. In Agrobacterium-mediated recombination the, plasmid ,construct must also contain T-DNA. Agrobacterium naturally inserts DNA from a
  15. Cells synthesize a complementary strand to produce a double-stranded circular, plasmid ,and also reproduce pile; both cells are now viable donors. The prototypical
  16. Artificially synthesized. Once isolated, the gene is inserted into a bacterial, plasmid , Constructs The gene to be inserted into the genetically modified organism must
  17. Moth caterpillars. In 1976,Robert A. Saharan reported the presence of a, plasmid ,in a strain of B. thuringiensis and suggested the plasmid 's involvement in
  18. Genome some of the donor’s chromosomal DNA may also be transferred with the, plasmid ,DNA. For example, the tumor-inducing (Ti) plasmid of Agrobacterium and the
  19. Are often referred to as the partition system or partition function of a, plasmid , Some plasmid s or microbial hosts include an addiction system or
  20. Exposed to the particular antibiotics. Only bacteria that take up copies of the, plasmid ,survive, since the plasmid makes them resistant. In particular, the protecting
  21. Called bactofection and in particular uses Agrobacterium tumefaciens. The, plasmid ,may be integrated into the genome, resulting in a stable transfection, or may
  22. Cell provides a conjugate or mobilizable genetic element that is most often a, plasmid ,or transpose. Most conjugate plasmid s have systems ensuring that the
  23. Vaccine therapy) applications. Daughter cells that retain a copy of the, plasmid ,survive, while a daughter cell that fails to inherit the plasmid dies or
  24. The first transgenic organism by inserting antibiotic resistance genes into the, plasmid ,of an E. coli bacterium. A year later Rudolf Jewish created a transgenic
  25. That only one of them survives in the cell line, due to the regulation of vital, plasmid ,functions. Therefore, plasmid s can be assigned into compatibility groups.
  26. Protein it then codes for, for example, insulin or even antibiotics. However,a, plasmid ,can contain inserts of only about 1–10 KB. To clone longer lengths of DNA
  27. Reproduce pile; both cells are now viable donors. The prototypical conjugate, plasmid ,is the F- plasmid , or F-factor. If the F- plasmid that is transferred has
  28. PSK),such as the HOK/so (host killing/suppressor of killing) system of, plasmid ,R1 in Escherichia coli. This variant produces both a long-lived poison and a
  29. In the cell is almost entirely determined by the rate of division of the, plasmid ,– fast division causes high copy number, and vice versa. Karyotype In general
  30. Resistance is an important tool for genetic engineering. By constructing a, plasmid ,which contains an antibiotic resistance gene as well as the gene being
  31. In both E. Cold and Agrobacterium. Once the binary vector is constructed the, plasmid ,is transformed into Agrobacterium containing no plasmid s and plant cells are
  32. Replicate along with it fairly harmlessly, or may even become established as a, plasmid , The virus remains dormant until host conditions deteriorate, perhaps due to
  33. Enhancer — operon **mutation — point mutation — crossover — recombination —, plasmid ,— transpose **molecular genetics: DNA fingerprinting — genetic fingerprint —
  34. 50S ribosomal subunit are rare. Chloramphenicol resistance may be carried on a, plasmid ,that also codes for resistance to other drugs. One example is the Accost plasmid
  35. Can ensure that when bacteria replicate, only the copies which carry along the, plasmid ,survive. This ensures that the gene being manipulated passes along when the
  36. Be transferred with the plasmid DNA. For example, the tumor-inducing (Ti), plasmid , of Agrobacterium and the root-tumor inducing (RI) plasmid of A. rhizomes
  37. A protein of interest is cloned (using PCR and/or restriction enzymes) into a, plasmid ,(known as an expression vector). A vector has 3 distinctive features: an
  38. Large amounts of proteins. In this case, researchers grow bacteria containing a, plasmid ,harboring the gene of interest. Just as the bacterium produces proteins to
  39. S genome it infects, causing crown gall disease. The T-DNA region of this, plasmid ,is responsible for insertion of the DNA. The genes to be inserted are cloned
  40. Also contain T-DNA. Agrobacterium naturally inserts DNA from a tumor inducing, plasmid ,into any susceptible plant's genome it infects, causing crown gall disease.
  41. Ti) plasmid of Agrobacterium and the root-tumor inducing (RI), plasmid , of A. rhizomes contain genes that are capable of transferring to plant cells.
  42. Animal cell to an electric shock, which can make the cell membrane permeable to, plasmid ,DNA. In some cases the electroporated cells will incorporate the DNA into their
  43. Produces both a long-lived poison and a short-lived antidote. Several types of, plasmid ,addiction systems (toxin/ antitoxin, metabolism-based,ORT systems) were
  44. Harvested, and lased (often using the alkaline lysis method) to isolate the, plasmid ,of interest. Another major use of plasmid s is to make large amounts of proteins
  45. From 1987 acquired greater significance. Notes In microbiology and genetics,a, plasmid ,is a DNA molecule that is separate from, and can replicate independently of
  46. Lactase is also used to screen for blue white colonies in the MCS of various, plasmid ,vectors in Escherichia coli or other bacteria. Linkin Park is an American rock
  47. A subset of the genes required for transfer. They can parasitize a conjugate, plasmid , transferring at high frequency only in its presence. Plasmids are now being
  48. Or lifetime). Rather than arising by mutation, they represent examples of, plasmid ,acquisition of beta-lactamase genes normally found on the chromosome of
  49. Isolated from a virulent strain of Enterobacter Hermógenes. It is carried on a, plasmid , pYMG-1,and is therefore transmissible to other bacterial strains. SME, IMI
  50. Plasmid that also codes for resistance to other drugs. One example is the Accost, plasmid ,(A ampicillin, C chloramphenicol, Co co-trimoxazole, T tetracycline),which

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